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Bioengineering

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JoVE Journal Bioengineering
Roterende Cell Culture Systems for Human Cell Culture
Imaging Integrin Tension and Cellular Force at Submicron Resolution with an Integrative Tension Sensor
Journal (Bioengineering)
Imaging Integrin Tension and Cellular Force at Submicron Resolution with an Integrative Tension Sensor
Measurement of Aggregate Cohesion by Tissue Surface Tensiometry
Journal (Bioengineering)
Measurement of Aggregate Cohesion by Tissue Surface Tensiometry
 
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Roterende Cell Culture Systems for Human Cell Culture: Human trofoblasten celler som en model

Article DOI: 10.3791/3367-v • 06:54 min • January 18th, 2012
January 18th, 2012 •
*1, *1,2, 3, 1, 1
1Department of Microbiology and Immunology, Tulane University Medical School, 2Physician/Scientist Program, Tulane University Medical School, 3Department of Molecular and Cellular Biology, Baylor College of Medicine
* These authors contributed equally

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Traditionel, todimensionale cellekultur teknikker ofte resultere i ændrede egenskaber med hensyn til differentiering markører, cytokiner og vækstfaktorer. Tre-dimensionel cellekultur i den roterende cellekultur-system (RCCS) genopretter udtryk for mange af disse faktorer, som vist her med en extravillous trofoblasten cellelinje.

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Bioteknik Extravillous trophoblasts cytotrophoblast invasion matrix metalloproteinase 3-D cellekultur RCCS ECM microcarriers
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