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Überwachung Kinase und Phosphatase-Aktivitäten durch den Zellzyklus durch Ratiometric FRET
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Überwachung Kinase und Phosphatase-Aktivitäten durch den Zellzyklus durch Ratiometric FRET

Article DOI: 10.3791/3410-v • 13:38 min • January 27th, 2012
January 27th, 2012 •
1, 1, 1, 1
1Department of Cell and Molecular Biology, Karolinska Institutet

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FRET-basierten Reporter werden immer häufiger eingesetzt, um Kinase-und Phosphatase-Aktivitäten in lebenden Zellen zu überwachen. Hier beschreiben wir eine Methode auf, wie FRET-basierten Reporter nutzen, um den Zellzyklus-abhängige Veränderungen in Ziel-Phosphorylierung zu beurteilen.

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Molecular Biology Ausgabe 59 FRET Kinase- Phosphatase lebenden Zellen Zellzyklus Mitose Plk1
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