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JoVE Journal
Biology

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通过细胞周期激酶和磷酸酶活性成比例的FRET监测
 
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通过细胞周期激酶和磷酸酶活性成比例的FRET监测

Article DOI: 10.3791/3410
January 27th, 2012

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Summary January 27th, 2012

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基于FRET的记者越来越多地用于监测活细胞中的激酶和磷酸酶的活动。在这里,我们描述了如何使用基于FRET的记者,以评估目标磷酸化细胞周期依赖性变化的方法。

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