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Overvågning kinase og fosfatasesæt aktiviteter gennem cellecyklus ved Ratiometrisk FRET
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Overvågning kinase og fosfatasesæt aktiviteter gennem cellecyklus ved Ratiometrisk FRET

Article DOI: 10.3791/3410-v • 13:38 min • January 27th, 2012
January 27th, 2012 •
1, 1, 1, 1
1Department of Cell and Molecular Biology, Karolinska Institutet

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FRET-baserede journalister i stigende grad anvendes til at overvåge kinase og fosfatase aktiviteter i levende celler. Her beskriver vi en metode til, hvordan du bruger FRET-baserede journalister til at vurdere cellecyklus-afhængige ændringer i mål fosforylering.

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Molekylær Biologi FRET kinase fosfatase levende celle cellecyklus mitose Plk1
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