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JoVE Journal Bioengineering
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Electric Field-controlled Directed Migration of Neural Progenitor Cells in 2D and 3D Environments

Article DOI: 10.3791/3453-v • 11:15 min • February 16th, 2012
February 16th, 2012 •
*1, *2,3, 1, 3, 3, 3, 1
1School of Dentistry, Cardiff Institute of Tissue Engineering & Repair, Cardiff University, 2Shandong Qianfoshan Hospital, Shandong University School of Medicine, 3Dermatology and Ophthalmology Research, Institute for Regenerative Cures, University of California at Davis
* These authors contributed equally

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Summary
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This protocol demonstrates methods used to establish 2D and 3D environments in custom-designed electrotactic chambers, which can track cells in vivo/ex vivo using time-lapse recording at the single cell level, in order to investigate galvanotaxis/electrotaxis and other cellular responses to direct current (DC) electric fields (EFs).

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Electric Field Neural Progenitor Cells 2D Environment 3D Environment Endogenous Electric Fields Tissue Development Tissue Regeneration Central Nervous System Spinal Cord Injuries Cell Migration Direct Current EFs Cell And Tissue Cultures Cellular Responses Single Cell Growth Conditions Organotypic Spinal Cord Slice Ex Vivo Model
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