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Journal / Biology / 使用网关兼容双分子荧光互补（附设）系统在植物蛋白相互作用检测…
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使用网关兼容双分子荧光互补（附设）系统在植物蛋白相互作用检测
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使用网关兼容双分子荧光互补（附设）系统在植物蛋白相互作用检测

Article DOI: 10.3791/3473 • 08:22 min
September 16th, 2011 •
1, 2, 2, 1, 2
1Department of Biology, University of Western Ontario, 2Southern Crop Protection and Food Research Centre, Agriculture and Agri-Food Canada

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September 16th, 2011

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我们已经开发出一种技术，以测试植物蛋白质相互作用。一个黄色荧光蛋白（YFP）被分成两个非重叠的片段。每个帧通过网关系统的兴趣基因片段克隆，使融合蛋白的表达。重组YFP的信号只发生在研讯的蛋白质相互作用。

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