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Detektion von Protein-Wechselwirkungen in Plant mit einem Gateway kompatibel bimolekularen Fluoreszenz Komplementation (BIFC) System

Article DOI: 10.3791/3473 • 08:22 min
September 16th, 2011 •
1, 2, 2, 1, 2
1Department of Biology, University of Western Ontario, 2Southern Crop Protection and Food Research Centre, Agriculture and Agri-Food Canada

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September 16th, 2011

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Wir haben eine Technik, um Protein-Protein-Interaktionen in Anlage-Test entwickelt. Ein gelb fluoreszierendes Protein (YFP) ist in zwei nicht überlappende Fragmente aufgeteilt. Jedes Fragment wird in-frame, ein Gen von Interesse via Gateway-System geklont, wodurch Expression von Fusionsproteinen. Rekonstitution der YFP-Signal erfolgt nur, wenn der gerichtlichen Untersuchung Proteinen interagieren.

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