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Rilevamento delle interazioni proteiche in pianta utilizzando un gateway compatibile complementazione fluorescenza biomolecolare (BiFC) Sistema

Article DOI: 10.3791/3473 • 08:22 min
September 16th, 2011 •
1, 2, 2, 1, 2
1Department of Biology, University of Western Ontario, 2Southern Crop Protection and Food Research Centre, Agriculture and Agri-Food Canada

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September 16th, 2011

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Abbiamo sviluppato una tecnica per testare interazioni proteina-proteina in pianta. Una proteina fluorescente gialla (YFP) è divisa in due frammenti non si sovrappongono. Ogni frammento viene clonato in-frame ad un gene di interesse attraverso il sistema Gateway, consentendo l'espressione di proteine ​​di fusione. Ricostituzione del segnale YFP si verifica solo quando le proteine ​​interagiscono inchiesta.

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