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Direct Detection of the Acetate-forming Activity of the Enzyme Acetate Kinase
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Direct Detection of the Acetate-forming Activity of the Enzyme Acetate Kinase

Article DOI: 10.3791/3474-v • 05:51 min • December 19th, 2011
December 19th, 2011 •
1, 1, 1
1Department of Genetics and Biochemistry, Clemson University

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Summary
Automatic Translation

A method for the determination of acetate kinase activity is described. This assay utilizes a direct reaction for determining enzyme activity and kinetics of acetate kinase in the acetate-forming direction with different phosphoryl acceptors. Furthermore, this method can be utilized for assaying other acetyl phosphate or acetyl-CoA utilizing enzymes.

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Acetate Kinase Acetate-forming Activity Enzyme Enzyme Superfamily Phosphorylation ATP Acetyl Phosphate Bacteria Archaea Eukarya Methanosarcina Thermophila Entamoeba Histolytica Amoebic Dysentery Hydroxamate Assay NADH NAD+ Pyruvate Kinase Lactate Dehydrogenase Inorganic Phosphate Hydroxylamine ATP Formation ADP NADP+ NADPH Hexokinase Glucose 6-phosphate Dehydrogenase
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