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Direkte påvisning af acetat-dannende aktivitet af enzymet Acetat Kinase
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Direkte påvisning af acetat-dannende aktivitet af enzymet Acetat Kinase

Article DOI: 10.3791/3474-v • 05:51 min • December 19th, 2011
December 19th, 2011 •
1, 1, 1
1Department of Genetics and Biochemistry, Clemson University

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En metode til bestemmelse af acetat kinase aktivitet er beskrevet. Denne analyse anvender en direkte reaktion til bestemmelse af enzymaktivitet og kinetik af acetat kinase i acetat-dannende retning med forskellige phosphoryl receptorer. Desuden kan metoden anvendes til analysering andre acetyl fosfat eller acetyl-CoA udnytte enzymer.

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Molekylær Biologi Acetat kinase acetat acetyl fosfat pyrophosphat PPI ATP
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