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Journal / Biology / Desplegable de proteínas de unión a calmodulina
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Desplegable de proteínas de unión a calmodulina

Article DOI: 10.3791/3502-v • 07:52 min • January 23rd, 2012
January 23rd, 2012 •
1, 1, 1, 1
1Department of Cell Biology, Neurobiology and Anatomy, Medical College of Wisconsin

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Calmodulina (CaM) pull-down de ensayo es una forma efectiva para investigar la interacción de la CAM con varias proteínas. Este método utiliza CAM-sepharose bolas para el análisis eficiente y específica de la CAM-proteínas de unión. Esta es una importante herramienta para explorar la señalización CAM en la función celular.

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Biología Molecular Número 59 calmodulina el calcio el CI-motivo la cromatografía de afinidad pull-down Ca2 + / calmodulina dependiente de quinasa II neurogranina
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