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Pull-down von Calmodulin-bindende Proteine
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Pull-down von Calmodulin-bindende Proteine

Article DOI: 10.3791/3502-v • 07:52 min • January 23rd, 2012
January 23rd, 2012 •
1, 1, 1, 1
1Department of Cell Biology, Neurobiology and Anatomy, Medical College of Wisconsin

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Calmodulin (CaM) Pull-Down-Assay ist ein effektiver Weg, um die Interaktion von CaM mit verschiedenen Proteinen zu untersuchen. Diese Methode verwendet CaM-Sepharose-Kügelchen für eine effiziente und spezifische Analyse von CaM-bindenden Proteinen. Dies stellt ein wichtiges Instrument zur CaM-Signalisierung in zelluläre Funktion zu erforschen.

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Molecular Biology Ausgabe 59 Calmodulin Kalzium IQ-Motiv Affinitätschromatographie pull-down Ca2 + / Calmodulin-abhängige Kinase II neurogranin
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