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Pull-down des protéines se liant à la calmoduline
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Pull-down des protéines se liant à la calmoduline

Article DOI: 10.3791/3502-v • 07:52 min • January 23rd, 2012
January 23rd, 2012 •
1, 1, 1, 1
1Department of Cell Biology, Neurobiology and Anatomy, Medical College of Wisconsin

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Calmoduline (CaM) pull-down test est un moyen efficace pour étudier l'interaction de la CAM avec diverses protéines. Cette méthode utilise la CaM-sépharose perles pour une analyse efficace et spécifique des protéines liant la CaM. Cela fournit un outil important d'explorer de signalisation CAM dans la fonction cellulaire.

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Biologie Moléculaire Numéro 59 la calmoduline le calcium le IQ-motif la chromatographie d'affinité pull-down Ca2 + / calmoduline-dépendante Kinase II neurogranine
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