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JoVE Journal
Biology

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Måling Fast Calcium Flux i cardiomyocytter
 
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Måling Fast Calcium Flux i cardiomyocytter

Article DOI: 10.3791/3505-v 12:11 min November 29th, 2011
November 29th, 2011

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Summary

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Vi præsenterer en metode til at isolere hurtig (mikrosekund) calcium begivenheder fra langsommere flusmidler i levende celler ved hjælp af laser scanning konfokal mikroskopi. Metoden foranstaltninger fluorescensintensitet udsving af calcium indikatorer ved at optage linje scanninger af flere hundrede pixel i en celle. Histogram analyse giver os mulighed for at isolere tidsskalaer af forskellige calcium flux.

Tags

Cellebiologi Calcium flux laserscanning mikroskopi cardiomyocytter fluorescerende indikatorer
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