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Journal / Biology / Måling Rask Kalsium flukser i cardiomyocytes
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Måling Rask Kalsium flukser i cardiomyocytes

Article DOI: 10.3791/3505-v • 12:11 min • November 29th, 2011
November 29th, 2011 •
1, 2
1Department of Biological Sciences and Geology, Queensborough Community College, 2Department of Physiology & Biophysics, Stony Brook University

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Vi presenterer en metode for å isolere rask (mikrosekund) kalsium hendelser fra tregere flukser i levende celler ved hjelp av laser scanning konfokalmikroskopi. Metoden måler fluorescensintensitet svingninger av kalsium indikatorer ved registrering linje skanninger av flere hundre piksler i en celle. Histogram analyse tillater oss å isolere tidsskalaer på forskjellige kalsium flukser.

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Cell Biology Kalsium flukser laser scanning mikroskopi cardiomyocytes fluorescerende indikatorer
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