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Bestemmelse af lipidklump opdeling af fluorescens-mærkede prober i levende celler ved fluorescenskorrelationsspektroskopi (FCS)
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Bestemmelse af lipidklump opdeling af fluorescens-mærkede prober i levende celler ved fluorescenskorrelationsspektroskopi (FCS)

Article DOI: 10.3791/3513-v • 10:59 min • April 6th, 2012
April 6th, 2012 •
1, 2,3, 1
1Centre de Recherche de l’Institut du Cerveau et de la Moelle Épinière, Hôpital de la Pitié-Salpêtrière, 2Institut des Sciences Moléculaires d'Orsay, Université Paris-Sud, 3Centre de Photonique Biomédicale du Centre Laser, Université Paris-Sud

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En teknik til at undersøge lipidklump opdelingen af ​​fluorescerende proteiner til plasmamembranen af ​​levende celler er beskrevet. Den udnytter forskellen i diffusionstider for proteiner i eller uden for lipidklumper. Erhvervelse kan udføres dynamisk i kontrol betingelser eller efter narkotika tilsætning.

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Cellular Biology lipidklumper plasmamembran diffusionstider konfokal mikroskopi
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