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Journal / Bioengineering / Spårning hypoxisk Signalering inom Inkapslade…
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Bioengineering

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Spårning hypoxisk Signalering inom Inkapslade cellaggregat

Article DOI: 10.3791/3521-v • 09:14 min • December 16th, 2011
December 16th, 2011 •
1, 1, 2
1Biomedical Engineering Program, University of South Carolina, 2Chemical Engineering Department, University of South Carolina

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En metod för foto-inkapsling av celler i en tvärbunden PEG hydrogel beskrivs. Hypoxiska signalering inom inkapslade murina insulinoma (MIN6) aggregat är spåras med hjälp av en fluorescerande markör system. Detta system möjliggör seriell undersökning av celler i en hydrogel klätterställning och korrelation av hypoxiska signalering med förändringar i cellernas fenotyp.

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Bioteknik Cell inkapsling PEG cell aggregering hypoxi insulinutsöndring lysrör bildhantering
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