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Journal / Biology / Intracellulære Refolding Assay
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JoVE Journal Biology
Intracellulære Refolding Assay
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Intracellulære Refolding Assay

Article DOI: 10.3791/3540-v • 07:19 min • January 24th, 2012
January 24th, 2012 •
1, 1
1Institute of Toxicology and Genetics, Karlsruhe Institute of Technology

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I denne protokollen en metode for å måle intracellulært protein refolding etter varmesjokkproteiner er beskrevet. Denne metoden kan brukes til å studere foldases som molekylær anstand og deres co-faktorer eller forbindelser i stand til å påvirke deres aktivitet. Firefly luciferase aktivitet brukes som reporter for å måle anstand refolding aktivitet.

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Molecular Biology anstand refolding stress luciferase varme sjokk
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