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Bioengineering
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光音響流量測定法を用いて循環メラノーマ細胞の検出と分離
JoVE Journal
Bioengineering
This content is Free Access.
JoVE Journal
Bioengineering
Detection and Isolation of Circulating Melanoma Cells using Photoacoustic Flowmetry
Please note that all translations are automatically generated.
Click here for the English version.
光音響流量測定法を用いて循環メラノーマ細胞の検出と分離
DOI:
10.3791/3559-v
•
09:52 min
•
November 25, 2011
•
Christine M. O’Brien
,
Kyle Rood
,
Shramik Sengupta
,
Sagar K. Gupta
,
Thiago DeSouza
,
Aaron Cook
,
John A. Viator
1
Biological Engineering, Christopher S. Bond Life Sciences Center, Dermatology
,
University of Missouri
Chapters
00:05
Title
01:20
Blood Sample Preparation
02:58
Flow Chamber Construction
04:32
Photoacoustic Flowmetry Set-up
06:42
Immunocytochemistry
08:39
Results: Melanoma Isolation
09:30
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我々は転移性疾患の早期指標として循環するメラノーマ細胞を検出するためにレーザー誘起超音波を用いてフローサイトメーターを開発しました。
Tags
Detection
Isolation
Circulating Melanoma Cells
Photoacoustic Flowmetry
Circulating Tumor Cells (CTCs)
Metastatic Disease
Blood Samples
Cancer Diagnosis
Therapy Response Monitoring
Pigment
Photoacoustic Waves
Custom Flow Cytometer
Separation
Centrifugation
White Blood Cell Layer
Density Similarity
Phosphate Buffered Saline (PBS)
Flowmeter
Two Phase Flow
Microfluidic System
Slugs Of Liquid
Laser Light Irradiation
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