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Expression Analys av däggdjur Linker-histon Subtyper

Article DOI: 10.3791/3577-v • 14:40 min • March 19th, 2012
March 19th, 2012 •
1, 1, 1, 1
1School of Biology and the Parker H. Petit Institute of Bioengineering and Biosciences, Georgia Institute of Technology

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Vi beskriver en uppsättning av analyser för att analysera expressionsnivåer av histoner H1 linker. mRNA av enskilda H1 gener kvantitativt mäts genom slumpmässig primer omvänd transkription följt av realtids-PCR, medan protein kvantifiering av H1 histoner uppnås genom HPLC-analys.

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Genetik H1 linker histoner histon H1 subtyper kromatin RT-PCR HPLC genuttryck
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