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Immunology and Infection

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JoVE Journal Immunology and Infection
Determining the Phagocytic Activity of Clinical Antibody Samples
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Determining the Phagocytic Activity of Clinical Antibody Samples

Article DOI: 10.3791/3588-v • 09:06 min • November 30th, 2011
November 30th, 2011 •
1, 1, 1, 1, 1, 2
1Massachusetts General Hospital, Ragon Institute of MGH, MIT, and Harvard, 2Thayer School of Engineering, Dartmouth College

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Summary
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We present a high-throughput flow cytometric assay to determine the phagocytic activity of antigen-specific antibodies from clinical samples, utilizing fluorescent antigen-coated beads and a monocytic cell line expressing multiple Fc receptors—providing receptor usage and phagocytic activity determinations in a standardized and reproducible fashion for any antigen of interest.

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Phagocytic Activity Clinical Antibody Samples Fc Receptors Clearance Pathology Of Disease Variable Domains Innate Immune Cells Recombinant Monoclonal Antibody Therapy Natural Infection Vaccination IgG Subclass Glycosylation Functional Differences Antigen-specific Antibodies Disease State Susceptibility To Infection Progression Clinical Outcome Effector Function Enhance Infection Fc Receptor-driven Phagocytosis Immune Complexes Th1/Th2 Polarization Assay
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