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JoVE Journal Bioengineering
Live-cell imaging att migrera celler som uttrycker fluorescerande-taggade proteiner i en tredimensionell matris
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Live-cell imaging att migrera celler som uttrycker fluorescerande-taggade proteiner i en tredimensionell matris

Article DOI: 10.3791/3589-v • 10:26 min • December 22nd, 2011
December 22nd, 2011 •
1, 1
1University of California, Davis

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Cellulära processer såsom cell migration har traditionellt studerats på två-dimensionell, styv plast ytor. Denna rapport beskriver en teknik för att direkt visualisera protein lokalisering och analys av proteiner dynamik i celler vandrar i ett mer fysiologiskt relevant tredimensionell matris.

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Bioteknik cell invasion tredimensionell matris kollagen gel live-cell konfokala imaging FRAP GFP epiteliala cystor
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