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Bioengineering

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JoVE Journal Bioengineering
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Live-cell Imaging of Migrering cellene uttrykker fluorescensmerket-merket Proteiner i en tredimensjonal Matrix

Article DOI: 10.3791/3589-v • 10:26 min • December 22nd, 2011
December 22nd, 2011 •
1, 1
1University of California, Davis

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Cellulære prosesser som celle migrasjon har tradisjonelt blitt studert på to-dimensjonale, stiv plast overflater. Denne rapporten beskriver en teknikk for direkte visualisere protein lokalisering og analyse av protein dynamikk i celler migrere i en mer fysiologisk relevant, tre-dimensjonal matrise.

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Bioteknologi celle invasjon tre-dimensjonal matrise kollagen gel live-cell confocal imaging FRAP GFP epitelial cyste
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