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Neuroscience

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JoVE Journal Neuroscience
Dissektion og Kultur af Chick Statoacoustic Ganglion og rygmarven eksplanteret i Kollagen Gels for neurite udvækst Analyser
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Dissektion og Kultur af Chick Statoacoustic Ganglion og rygmarven eksplanteret i Kollagen Gels for neurite udvækst Analyser

Article DOI: 10.3791/3600-v • 11:08 min • December 20th, 2011
December 20th, 2011 •
1, 1
1Department of Biological Sciences, Purdue University

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Vi viser, hvordan du dissekere og kultur chick E4 statoacoustic ganglion og E6 rygmarven eksplanteret. Eksplanteret dyrkes under serumfrit betingelser i 3D kollagen geler i 24 timer. Neurite lydhørhed er testet med vækstfaktor-suppleret medium og med protein-belagt perler.

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Neurovidenskab kylling dissektion morphogen NT-3 neurite outgrowth rygmarv statoacoustic ganglion Wnt5a
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