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Journal / Biology / In vivo Elektroporatie van Morpholinos in de…
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In vivo Elektroporatie van Morpholinos in de Adult zebravis Retina

Article DOI: 10.3791/3603-v • 06:55 min • December 27th, 2011
December 27th, 2011 •
1, 2,3, 2,3
1Departments of Anatomy and Cell Biology and Ophthalmology, Wayne State University School of Medicine, 2Department of Biological Sciences, University of Notre Dame, 3Center for Zebrafish Research, University of Notre Dame

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Een methode om een ​​doelwit eiwit expressie voorwaardelijk knockdown in de volwassen zebravissen netvlies wordt beschreven, wat inhoudt intravitreally injecteren van antisense morpholinos en electroporating ze in het netvlies. De daaruit resulterende eiwitten neergeslagen voor meerdere dagen, waardoor het testen van de eiwit rol in de regenereren of intact netvlies.

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Developmental Biology elektroporatie morfolino zebravis netvlies regeneratie
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