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In-vivo-Elektroporation von Morpholinos in die Adult Zebrafisch Retina

Article DOI: 10.3791/3603-v • 06:55 min • December 27th, 2011
December 27th, 2011 •
1, 2,3, 2,3
1Departments of Anatomy and Cell Biology and Ophthalmology, Wayne State University School of Medicine, 2Department of Biological Sciences, University of Notre Dame, 3Center for Zebrafish Research, University of Notre Dame

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Eine Methode, bedingt Knockdown ein Zielprotein Ausdruck in der erwachsenen Zebrafisch Retina beschrieben, das geht intravitreal Injektion antisense Morpholinos und Elektroporation sie in der Netzhaut. Das resultierende Protein ist für mehrere Tage, die Prüfung des Proteins Rolle bei der Regeneration oder intakten Netzhaut ermöglicht klopfte.

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Developmental Biology Ausgabe 58 Elektroporation Morpholino Zebrafisch Netzhaut Regeneration
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