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Immunology and Infection

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JoVE Journal Immunology and Infection
High-throughput Detection Methode voor Influenza Virus
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High-throughput Detection Methode voor Influenza Virus

Article DOI: 10.3791/3623-v • 10:05 min • February 4th, 2012
February 4th, 2012 •
1, 1, 2, 3, 4, 4, 1,5, 1,6
1Laboratory of Molecular Immunology and Immunotherapy, Blood Research Institute, 2Department of Microbiology, Mount Sinai School of Medicine, 3Laboratory of Molecular Genetics, Blood Research Institute, 4City of Milwaukee Health Department Laboratory, 5Division of Hematology-Oncology/BMT, Children's Hospital of Wisconsin, Medical College of Wisconsin, 6Division of Hematology and Oncology, Dept Medicine, Medical College of Wisconsin

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Deze methode beschrijft het gebruik van Infrared kleurstofinkt imaging systeem voor detectie van H1N1 in bronchioalveolar lavage (BAL) vloeistof van geïnfecteerde muizen op een hoog gevoeligheid. Deze methodologie kan worden uitgevoerd in een 96 - of 384-wells plaat, vereist <10 pi volume van testmateriaal en heeft het potentieel voor gelijktijdige screening van meerdere pathogenen.

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Immunologie Influenza virus virus titer epitheelcellen
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