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JoVE Journal
Biology

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In vivo elektroporation af Morpholinos i Regenerating Adult zebrafisk Tail Fin
 
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In vivo elektroporation af Morpholinos i Regenerating Adult zebrafisk Tail Fin

Article DOI: 10.3791/3632
March 29th, 2012

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Summary March 29th, 2012

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Click here for the English version.

Vi beskriver en fremgangsmåde til konditionelt knockdown ekspressionen af ​​et målprotein i voksne zebrafisk fin regenerering. Denne teknik involverer mikro-injektion, og elektroporering antisense oligonucleotid morpholinos i fin væv, som tillader afprøvning af proteinets rolle i forskellige stadier af fin regenerering, herunder sårheling, blastema dannelse og regenerative udvækst.

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