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In-vivo-Elektroporation von Morpholinos in das Regenerations Adult Zebrafisch Leitwerk
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In-vivo-Elektroporation von Morpholinos in das Regenerations Adult Zebrafisch Leitwerk

Article DOI: 10.3791/3632-v • 07:22 min • March 29th, 2012
March 29th, 2012 •
1, 2, 3
1Department of Biological Sciences, Center for Zebrafish Research, University of Notre Dame, 2Department of Microbiology, Immunology, and Pathology, Colorado State University, 3Departments of Anatomy and Cell Biology and Ophthalmology, Wayne State University School of Medicine

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Wir beschreiben ein Verfahren zur bedingten Knockdown die Expression eines Zielproteins im Erwachsenenalter Zebrafisch Flossenregeneration. Diese Technik beinhaltet Mikrospritzvorrichtung und Elektroporation Antisense-Oligonukleotid in Morpholinos Flossengewebes das Testen des Proteins Rolle in verschiedenen Stadien der Flossenregeneration, einschließlich Wundheilung, Blastembildung und regenerative Auswachsen ermöglicht.

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Developmental Biology Ausgabe 61 die Elektroporation Morpholino Zebrafisch FIN Regeneration
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