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JoVE Journal
Immunology and Infection

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Assessing Hepatic Metabolic Changes During Progressive Colonization of Germ-free Mouse by 1H NMR Spectroscopy
 

Assessing Hepatic Metabolic Changes During Progressive Colonization of Germ-free Mouse by 1H NMR Spectroscopy

Article DOI: 10.3791/3642-v 07:54 min December 15th, 2011
December 15th, 2011

Chapters

Summary

A progressive colonization procedure is described to further assess its impact on the host hepatic metabolism. Colonization is monitored non invasively by evaluating the urinary excretion of microbial co-metabolites by NMR-based metabolic profiling while hepatic metabolism is assessed by High Resolution Magic Angle Spinning (HR MAS) NMR profiling of intact biopsy.

Tags

Hepatic Metabolic Changes Germ-free Mouse 1H NMR Spectroscopy Gut Bacteria Host Homeostasis Immune Protection Vitamin Synthesis Gut Microbiota Metabolic Interaction Colonization Process Normalization Process Gavage Method Bacterial Establishment
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