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JoVE Journal
Biology
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Identifikation af Protein Interagerende Partners Brug Tandem Affinitetsrensning
 
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Identifikation af Protein Interagerende Partners Brug Tandem Affinitetsrensning

Article DOI: 10.3791/3643
February 25th, 2012

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Summary February 25th, 2012

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Click here for the English version.

Tandem affinitetsoprensning er en robust fremgangsmåde til identifikation af protein-bindende partnere. Som proof of concept, blev denne metode anvendt på velkarakteriserede translationsstart faktor eIF4E til co-bundfald værtscellesystemerne faktorer involveret i oversættelse initiering. Denne fremgangsmåde kan let tilpasses til enhver cellulært eller viralt protein.

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