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JoVE Journal Immunology and Infection
Påvisning af toksin translokation i værtslandet cytosolen af ​​overflade plasmon resonans
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Påvisning af toksin translokation i værtslandet cytosolen af ​​overflade plasmon resonans

Article DOI: 10.3791/3686-v • 10:41 min • January 3rd, 2012
January 3rd, 2012 •
1, 1, 1, 1
1Department of Molecular Biology and Microbiology, University of Central Florida

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I denne rapport beskriver vi, hvordan overfladen plasmon resonans bruges til at påvise toksinet indrejse i værten cytosol. Denne meget følsom metode kan give kvantitative data om mængden af ​​cytosole toksin, og det kan anvendes til en række af toksiner.

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Immunologi Overflade plasmon resonans AB toksin translokation endoplasmatiske reticulum cellekultur kolera toksin pertussistoksin
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