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Immunology and Infection

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JoVE Journal Immunology and Infection
Nachweis von Toxin Translokation in den Host-Cytosol von Surface Plasmon Resonance
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Nachweis von Toxin Translokation in den Host-Cytosol von Surface Plasmon Resonance

Article DOI: 10.3791/3686-v • 10:41 min • January 3rd, 2012
January 3rd, 2012 •
1, 1, 1, 1
1Department of Molecular Biology and Microbiology, University of Central Florida

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In diesem Bericht beschreiben wir, wie Oberflächen-Plasmon-Resonanz verwendet wird, um Toxin Einreise in den Aufnahmemitgliedstaat Zytosol zu erkennen. Dieser hochempfindliche Methode lassen sich quantitative Daten über die Höhe der zytosolischen Toxin geben, und es kann zu einer Reihe von Toxinen eingesetzt werden.

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Immunologie Ausgabe 59 Surface Plasmon Resonance AB-Toxin Translokation endoplasmatisches Retikulum Zellkultur Cholera-Toxin Pertussis-Toxin
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