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Immunology and Infection

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JoVE Journal Immunology and Infection
Påvisning av giftstoffer translokasjon i Host cytosol ved Surface Plasmon Resonance
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Påvisning av giftstoffer translokasjon i Host cytosol ved Surface Plasmon Resonance

Article DOI: 10.3791/3686-v • 10:41 min • January 3rd, 2012
January 3rd, 2012 •
1, 1, 1, 1
1Department of Molecular Biology and Microbiology, University of Central Florida

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I denne rapporten beskriver vi hvordan overflaten plasmon resonans blir brukt til å påvise toksin innreise til verten cytosol. Denne svært følsom metode kan gi kvantitative data om mengden av cytosoliske toksin, og den kan brukes til en rekke giftstoffer.

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Immunologi Surface plasmon resonans AB toksin translokasjon endoplasmatiske retikulum cellekultur koleratoksin pertussis toksin
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