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JoVE Journal
Bioengineering

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网站特定的细菌染色体工程:ΦC​​31整合介导的盒式交换(IMCE)
 
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网站特定的细菌染色体工程:ΦC​​31整合介导的盒式交换(IMCE)

Article DOI: 10.3791/3698
March 16th, 2012

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Summary March 16th, 2012

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描述一个快速和有效的方法融入预制受体菌株,称为停机坪株,外源DNA的利益。该方法允许进入工程着陆垫一个给定的应变轨迹的站点特定的DNA盒的整合,通过共轭ΦC31整合表达。

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