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Journal / Bioengineering / 网站特定的细菌染色体工程：ΦC​​31整合介导的盒式交换（IMCE）…
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Bioengineering

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JoVE Journal Bioengineering
网站特定的细菌染色体工程：ΦC​​31整合介导的盒式交换（IMCE）
Magnetic Resonance Elastography Methodology for the Evaluation of Tissue Engineered Construct Growth
Journal (Bioengineering)
Magnetic Resonance Elastography Methodology for the Evaluation of Tissue Engineered Construct Growth
Correlative Light Electron Microscopy (CLEM) for Tracking and Imaging Viral Protein Associated Structures in Cryo-immobilized Cells
Journal (Bioengineering)
Correlative Light Electron Microscopy (CLEM) for Tracking and Imaging Viral Protein Associated Structures in Cryo-immobilized Cells
 
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网站特定的细菌染色体工程：ΦC​​31整合介导的盒式交换（IMCE）

Article DOI: 10.3791/3698-v • 08:21 min • March 16th, 2012
March 16th, 2012 •
1, 1, 1
1Biology, University of Waterloo

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描述一个快速和有效的方法融入预制受体菌株，称为停机坪株，外源DNA的利益。该方法允许进入工程着陆垫一个给定的应变轨迹的站点特定的DNA盒的整合，通过共轭ΦC31整合表达。

Tags

生物工程，第61期，ΦC31整合酶，Rhizobi​​ales，染色体工程，细菌遗传学
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