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Journal / Bioengineering / 网站特定的细菌染色体工程：ΦC​​31整合介导的盒式交换（IMCE）…
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JoVE Journal Bioengineering
网站特定的细菌染色体工程：ΦC​​31整合介导的盒式交换（IMCE）
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网站特定的细菌染色体工程：ΦC​​31整合介导的盒式交换（IMCE）

Article DOI: 10.3791/3698 • 08:21 min
March 16th, 2012 •
1, 1, 1
1Biology, University of Waterloo

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March 16th, 2012

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描述一个快速和有效的方法融入预制受体菌株，称为停机坪株，外源DNA的利益。该方法允许进入工程着陆垫一个给定的应变轨迹的站点特定的DNA盒的整合，通过共轭ΦC31整合表达。

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