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JoVE Journal
Bioengineering

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Site-specifik bakteriekromosomet Engineering
 

Site-specifik bakteriekromosomet Engineering: ΦC31 Integrase Mediated Cassette Exchange (IMCE)

Article doi: 10.3791/3698
March 16th, 2012

Summary March 16th, 2012

Please note that all translations are automatically generated.

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En hurtig og effektiv metode til at integrere fremmed DNA af interesse i præfremstillede acceptor stammer, betegnet landingsplads stammer, er beskrevet. Fremgangsmåden tillader stedspecifik integration af et DNA-kassette i det konstruerede landingsplads locus af en given stamme, gennem konjugation og ekspression af det ΦC31 integrase.

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