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Journal / Bioengineering / Stedsspesifikk Bakteriell Chromosome Engineering: …
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Bioengineering

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JoVE Journal Bioengineering
Stedsspesifikk Bakteriell Chromosome Engineering
Isolation of Cellular Lipid Droplets: Two Purification Techniques Starting from Yeast Cells and Human Placentas
Journal (Bioengineering)
Isolation of Cellular Lipid Droplets: Two Purification Techniques Starting from Yeast Cells and Human Placentas
Double Emulsion Generation Using a Polydimethylsiloxane (PDMS) Co-axial Flow Focus Device
Journal (Bioengineering)
Double Emulsion Generation Using a Polydimethylsiloxane (PDMS) Co-axial Flow Focus Device
 
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Stedsspesifikk Bakteriell Chromosome Engineering: ΦC31 integrase Mediated Cassette Exchange (IMCE)

Article DOI: 10.3791/3698-v • 08:21 min • March 16th, 2012
March 16th, 2012 •
1, 1, 1
1Biology, University of Waterloo

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En rask og effektiv metode for å integrere fremmed DNA av interesse i pre-laget akseptor stammer, betegnes Matte stammer, er beskrevet. Metoden gjør at stedsspesifikk integrering av en DNA kassetten inn i konstruert landing puten locus av en gitt belastning, gjennom konjugering og uttrykk for ΦC31 integrase.

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Bioteknologi ΦC31 integrase Rhizobiales Kromosom Engineering bakterielle genetikk
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