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Immunology and Infection

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Generation of Induced Regulatory T Cells from Primary Human Naïve and Memory T Cells

Article DOI: 10.3791/3738 • 14:24 min
April 16th, 2012 •
1, 1, 1, 1, 1
1Department of Microbiology, Immunology and Molecular Genetics, University of Kentucky

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April 16th, 2012

We describe a method for generating regulatory, memory and naïve T cells from a single human blood donor. Polarized Tregs can be then compared to other subsets in a variety of genetic and functional applications with genetic homogeneity, including a suppression assay also detailed here.

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