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Journal / Neuroscience / Laterale diffusie en Exocytose van…
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Neuroscience

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JoVE Journal Neuroscience
Laterale diffusie en Exocytose van membraaneiwitten in gekweekte zenuwcellen gemeten met behulp van Fluorescence Recovery en fluorescentie-verlies fotobleken
Studying Pre-formed Fibril Induced α-Synuclein Accumulation in Primary Embryonic Mouse Midbrain Dopamine Neurons
Journal (Neuroscience)
Studying Pre-formed Fibril Induced α-Synuclein Accumulation in Primary Embryonic Mouse Midbrain Dopamine Neurons
Cerebral Blood Oxygenation Measurement Based on Oxygen-dependent Quenching of Phosphorescence
Journal (Neuroscience)
Cerebral Blood Oxygenation Measurement Based on Oxygen-dependent Quenching of Phosphorescence
 
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Laterale diffusie en Exocytose van membraaneiwitten in gekweekte zenuwcellen gemeten met behulp van Fluorescence Recovery en fluorescentie-verlies fotobleken

Article DOI: 10.3791/3747-v • 11:59 min • February 29th, 2012
February 29th, 2012 •
1, 1, 1, 1
1MRC Centre for Synaptic Plasticity, University of Bristol

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Dit rapport beschrijft het gebruik van live cell imaging en photobleach technieken om de oppervlakte-expressie, transportroutes en handel in kinetiek van exogeen uitgedrukt, pH-gevoelige GFP-gelabelde eiwitten in de plasmamembraan van neuronen te bepalen.

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Neuroscience Fluorescence Recovery After Photobleaching FRAP confocale beelden fluorofoor GFP Super-ecliptica pHluorin SEP fluorescentie verlies in photobleach FLIP neuron eiwit verkeer synaps
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