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Journal / Neuroscience / Kültür Nöronlar Membran Proteinlerin Yanal…
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Neuroscience

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JoVE Journal Neuroscience
Kültür Nöronlar Membran Proteinlerin Yanal Difüzyon ve ekzositoz Floresans Kurtarma ve Floresans kaybı Photobleaching kullanarak Değerlendirilen
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Kültür Nöronlar Membran Proteinlerin Yanal Difüzyon ve ekzositoz Floresans Kurtarma ve Floresans kaybı Photobleaching kullanarak Değerlendirilen

Article DOI: 10.3791/3747-v • 11:59 min • February 29th, 2012
February 29th, 2012 •
1, 1, 1, 1
1MRC Centre for Synaptic Plasticity, University of Bristol

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Bu rapor yüzey ekspresyonu, ulaşım yolları ve dışsal olarak ifade edilen, pH-duyarlı GFP-etiketli nöronların plazma membran proteinlerinin kaçakçılığı kinetik belirlemek için canlı hücre görüntüleme ve photobleach tekniklerinin kullanımı anlatılmaktadır.

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Nörobilim Sayı 60 sonra Floresans Kurtarma FRAP FLIP Konfokal görüntüleme fluorofor GFP Super-ekliptik pHluorin SEP photobleach floresans kaybı nöron protein trafiğinin sinaps Photobleaching
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