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记录神经反应和注射示踪剂一个清醒小鼠的制备
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Neuroscience
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JoVE Journal Neuroscience
Preparation of an Awake Mouse for Recording Neural Responses and Injecting Tracers
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记录神经反应和注射示踪剂一个清醒小鼠的制备

DOI:
10.3791/3755-v

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14:06 min

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June 26, 2012

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, , ,
1Department of Neuroscience,Johns Hopkins University, 2Garvan Institute of Medical Research, 3School of Biological Sciences,Washington State University, 4Department of Otolaryngology-HNS,Johns Hopkins University

Chapters

  • 00:05Title
  • 01:54Preparation of the Head Restraint
  • 04:14Stereotaxic Alignment for Craniotomy
  • 08:24Head-post and Ground Pin Installation
  • 10:20Craniotomy
  • 11:54Results: Electrophysiological Recordings and Tracing Studies of the Inferior Colliculus
  • 13:19Conclusion

Summary

Automatic Translation

Automatic Translation

重要的是理解大脑功能和指导安置途径示踪染料神经反应的电特性。然而，许多研究都在麻醉动物。理解没有麻醉药的脑功能，我们开发了一种方法来记录神经元的反应特性，并在清醒小鼠注入染料。

Tags

Awake MouseRecording Neural ResponsesInjecting TracersAnesthesiaBrainstem NeuronsFundamental Response PropertiesDecerebrate PreparationElectrode ArraysChronic RecordingAwake And Behaving AnimalsTracer DyesHead Restraint Technique

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