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Immunology and Infection

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JoVE Journal Immunology and Infection
Single-celle Analyse av Bacillus subtilis Biofilm Bruke fluorescensmikroskopi og flowcytometrisystemer
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Single-celle Analyse av Bacillus subtilis Biofilm Bruke fluorescensmikroskopi og flowcytometrisystemer

Article DOI: 10.3791/3796-v • 13:28 min • February 15th, 2012
February 15th, 2012 •
1, 1, 1, 1
1Institute for Molecular Infection Biology (IMIB), University of Würzburg

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Mikrobielle biofilmer er generelt konstituert av distinkte subpopulasjoner av spesialiserte celler. Single-celle analyse av disse subpopulasjoner krever bruk av fluorescerende reportere. Her beskriver vi en protokoll for å visualisere og overvåke flere subpopulationswithin

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Immunologi , Biofilmdannelse genekspresjon celledifferensiering encellet analyse
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