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Immunology and Infection

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JoVE Journal Immunology and Infection
Engineering en Evolutie van synthetische adeno-geassocieerd virus (AAV) gentherapievectoren via DNA Family Shuffling
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Engineering en Evolutie van synthetische adeno-geassocieerd virus (AAV) gentherapievectoren via DNA Family Shuffling

Article DOI: 10.3791/3819-v • 21:56 min • April 2nd, 2012
April 2nd, 2012 •
*1, *1, 2, 1, 1, 1, 1
1Cluster of Excellence CellNetworks, Department of Infectious Diseases, Virology, Heidelberg University, 2Department of Infectious Diseases, Virology, Heidelberg University
* These authors contributed equally

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We tonen de basistechniek om moleculair ingenieur en synthetische adeno-geassocieerde virale (AAV) gentherapievectoren evolueren via DNA-familie shuffelen. Bovendien geven we algemene richtlijnen en representatieve voorbeelden voor de selectie en analyse van individuele chimere capsiden met verbeterde eigenschappen op doel cellen in cultuur of in muizen.

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Immunology Nummer 62 Adeno-associated virus AAV gentherapie synthetische biologie virale vector moleculaire evolutie van DNA-shuffelen
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