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Immunology and Infection

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JoVE Journal Immunology and Infection
Engineering and Evolution of Synthetic Adeno-assosiert virus (AAV) genterapi vektorer via DNA Family shuffling
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Engineering and Evolution of Synthetic Adeno-assosiert virus (AAV) genterapi vektorer via DNA Family shuffling

Article DOI: 10.3791/3819-v • 21:56 min • April 2nd, 2012
April 2nd, 2012 •
*1, *1, 2, 1, 1, 1, 1
1Cluster of Excellence CellNetworks, Department of Infectious Diseases, Virology, Heidelberg University, 2Department of Infectious Diseases, Virology, Heidelberg University
* These authors contributed equally

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Vi viser den grunnleggende teknikken til å molecularly konstruere og utvikle syntetiske Adeno-tilknyttede viral (AAV) genterapi vektorer via DNA familie shuffling. Videre gir vi generelle retningslinjer og representative eksempler for utvalg og analyse av individuelle chimeric capsids med forbedrede egenskaper på målceller i kultur eller i mus.

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Immunologi Adeno-assosiert virus AAV genterapi syntetisk biologi viral vektor molekylær evolusjon DNA shuffling
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