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JoVE Journal Neuroscience
Multiphoton Mikroskopi av Slettet Mouse Brain Uttrykke YFP
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Multiphoton Mikroskopi av Slettet Mouse Brain Uttrykke YFP

Article DOI: 10.3791/3848-v • 10:04 min • September 23rd, 2012
September 23rd, 2012 •
*1, *1, 1,2, 1
1Department of Biomedical Engineering, Yale University, 2Department of Biomedical Engineering, Louisiana Tech University
* These authors contributed equally

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Multiphoton mikroskopi av hele mus organer er mulig ved optisk fjerne organ før bildebehandling, men ikke alle protokoller bevare den fluorescerende signal av fluorescerende proteiner. Bruke en optisk clearing metoden med etanol-basert dehydrering og benzylalkohol: benzylbenzoat clearing, viser vi høyoppløselige multiphoton bilder av hele mus hjernen uttrykker YFP.

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Nevrovitenskap Biomedical Engineering molekylær biologi multiphoton mikroskopi mus hjerne clearing YFP fluroescence
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