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Multiphotonen Mikroskopie Cleared Maushirn Ausdruck YFP
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Multiphotonen Mikroskopie Cleared Maushirn Ausdruck YFP

Article DOI: 10.3791/3848-v • 10:04 min • September 23rd, 2012
September 23rd, 2012 •
*1, *1, 1,2, 1
1Department of Biomedical Engineering, Yale University, 2Department of Biomedical Engineering, Louisiana Tech University
* These authors contributed equally

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Mehrphotonenmikroskopie ganzer Mausorganen ist durch optisches Löschen des Organs vor der Bildgebung möglich, aber nicht alle Protokolle bewahren das Fluoreszenzsignal von fluoreszierenden Proteinen. Unter Verwendung eines optischen Clearing-Methode mit Ethanol-basierte Austrocknung und Benzylalkohol: Benzylbenzoat Lichtung zeigen wir, hochauflösende Multiphotonen Bilder gesamte Gehirn der Maus zum Ausdruck YFP.

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Neuroscience Ausgabe 67 Biomedical Engineering Molekularbiologie Multiphotonen-Mikroskopie Maus Gehirn- Clearing- YFP fluroescence
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