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JoVE Journal Bioengineering
Mikropipette Aspirasjon av substrat-tilkoblede celler å anslå Cell Stivhet
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Mikropipette Aspirasjon av substrat-tilkoblede celler å anslå Cell Stivhet

Article DOI: 10.3791/3886-v • 10:31 min • September 27th, 2012
September 27th, 2012 •
1, 1, 2, 1
1Section of Respiratory, Critical Care and Sleep Medicine, Department of Medicine, University of Illinois, 2Institute for Medicine and Engineering, University of Pennsylvania

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Her beskriver vi en rask og enkel metode for å måle celle stivhet. Det generelle prinsipp ved denne tilnærmingen er å måle membran deformasjon i respons til veldefinerte undertrykk påføres gjennom en mikropipette til celleoverflaten. Denne metoden gir et kraftig verktøy for å studere biomekaniske egenskaper substrat-tilkoblede celler.

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Bioteknologi biofysikk Biomedical Engineering medisin cellebiologi Cell stivhet biomekanikk microaspiration cellemembranen cytoskjelettet
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