JoVE Journal
Biology
Biology
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亲和沉淀主动RHO上皮细胞裂解液中使用的GST标签的突变Rho蛋白(GST-RhoA蛋白(G17A))全环基金
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Article
DOI:
10.3791/3932-v
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11:29 min
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March 31st, 2012
亲和沉淀主动RHO上皮细胞裂解液中使用的GST标签的突变Rho蛋白(GST-RhoA蛋白(G17A))全环基金
March 31st, 2012
•
Chapters
Summary
Please note that all translations are automatically generated.
Click here for the English version.
这里介绍的方法检测,按照激活RhoA的具体GDP / GTP交换因子(全环基金)在培养细胞中的突变RhoA的GST融合蛋白具有高亲和力激活全环基金的使用。全环基金沉淀的细胞裂解物,免疫印迹和密度定量检测。