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JoVE Journal Biology
Protein-Protein Etkileşimi Okumak Manyetik Boncuk Kullanarak Bir Sıvı Faz Affinity Yakalama Testi
Examining the Conformational Dynamics of Membrane Proteins in situ with Site-directed Fluorescence Labeling
Journal (Biology)
Examining the Conformational Dynamics of Membrane Proteins in situ with Site-directed Fluorescence Labeling
Analyzing the α-Actinin Network in Human iPSC-Derived Cardiomyocytes Using Single Molecule Localization Microscopy
Journal (Biology)
Analyzing the α-Actinin Network in Human iPSC-Derived Cardiomyocytes Using Single Molecule Localization Microscopy
 
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Protein-Protein Etkileşimi Okumak Manyetik Boncuk Kullanarak Bir Sıvı Faz Affinity Yakalama Testi: Poliovirüs-Nanobody Örnek

Article DOI: 10.3791/3937
May 29th, 2012 •
1, 1, 1
1Department of Pharmaceutical Biotechnology and Molecular Biology, Centre for Pharmaceutical Research, Vrije Universiteit Brussel

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May 29th, 2012

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Bu makalede, basit, kantitatif, sıvı faz afinitesi yakalama deneyi sunulmuştur. Bu bir yandan, diğer tarafta etiketli protein ve ikinci, etiketli protein (örn. poliovirus) arasındaki yakınlığı manyetik boncuk ve etiketli proteinlerin (örneğin nanobodies) arasındaki etkileşime dayanan güvenilir bir tekniktir.

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