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Nachweis seltener Varianten von Genomic Pooled-Sequenzierung mit SPLINTER
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Nachweis seltener Varianten von Genomic Pooled-Sequenzierung mit SPLINTER

Article DOI: 10.3791/3943-v • 14:06 min • June 23rd, 2012
June 23rd, 2012 •
1, 1, 2, 1, 1,3
1Center for Genome Sciences and Systems Biology, Department of Genetics, Washington University School of Medicine, 2Department of Internal Medicine, Washington University School of Medicine, 3Department of Pediatrics, Washington University School of Medicine

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Pooled DNA-Sequenzierung ist eine schnelle und kostengünstige Strategie, um seltene Varianten mit komplexen Phänotypen assoziiert in großen Kohorten zu erkennen. Hier beschreiben wir die computergestützte Analyse von gepoolten, Next-Generation-Sequenzierung von 32 Krebs-Genen unter Verwendung des SPLINTER Software-Paket. Diese Methode ist skalierbar und für jeden interessierenden Phänotyp.

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Genetik Genomics Cancer Biology Bioinformatics Pooled DNA-Sequenzierung Splinter seltenen genetischen Varianten genetisches Screening Phänotyp hoher Durchsatz computergestützte Analyse DNA PCR-Primer
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